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KMID : 1377020130100040176
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Tissue Engineering and Regenerative Medicine
2013 Volume.10 No. 4 p.176 ~ p.182
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The effect of fibroblast growth factor and periodontal ligament fibroblast-conditioned medium on fibroblast-related gene expression in bone marrow stromal cells
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Seo Young-Jong
Lee IL-kwon
Jang Hyun-Seon
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Abstract
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Periodontal ligament fibroblasts (PDLFs) are essential to most periodontal tissue regeneration processes. This study evaluated the potential of fibroblast growth factor (FGF) and PDLF-conditioned media to modulate fibroblast differentiation from bone marrow stromal cells (BMSCs) that were obtained from mongrel dogs. The medium obtained from culture of periodontal ligament fibroblasts of dogs was used for the PDLF-conditioned media. In the control group, the BMSCs were only cultured without FGF and PDLF-conditioned media. In experimental group 1, the BMSCs were loaded with FGF (3 ng/mL). In experimental group 2, the BMSCs were treated with PDLF-conditioned media. The BMSCs in experimental group 3 were loaded with FGF (3 ng/mL) and PDLF-conditioned media. The media was changed every 2 days. The cells of the control and experimental groups were cultured for 1, 11 and 14 days. The effects of the FGF and PDLF-conditioned media on fibroblast-related gene expression from the bone marrow stromal cells were examined by reverse transcription-polymerase chain reaction. Intensity of each band of the genes expressed was evaluated. The addition of FGF and PDLF-conditioned media enhanced the differentiation of fibroblast like cells from bone marrow stromal cell. The experimental group-treated FGF and PDLFconditioned media showed the strong expression of the fibroblast cell-specific marker: UNCL-, S100A2- and S100A4-mRNA at 14 days. Strong nestin mRNA expression was observed in the control group. This study suggests that FGF and PDLF-conditioned medium can enhance fibroblast-related gene expression in BMSCs in vitro.
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KEYWORD
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BMSC, FGF, gene, fibroblast, differentiation
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